Volume EM

In Volume EM (vEM or 3D EM) a reconstructed 3D image of intracellular ultrastructural detail can be formed using sequential SEM images of cross-sections through a sample. The possible sample volume imaged in this way is greater than normally possible 2D thin section imaging using TEM.

The Wolfson Bioimaging Facility has a growing capacity to support different volume EM approaches with its high specification SEM.

Serial block face scanning electron microscopy (SBF-SEM) with Zeiss Gemini SEM 450/Gatan 3View.
Coming soon: Array Tomography (AT) using Zeiss Gemini SEM 450/ASTEM detector.

More information and access

For further information or to arrange access to this equipment, contact one of the team.

Please fill in this form to register sample processing requests.

We welcome comments or suggestions. Please contact one of the team or one of the steering group.