The Wolfson Bioimaging Facility has a suite of widefield microscopes offering routine fluorescence and transmitted light imaging alongside advanced applications including ratiometric analysis (e.g. FRET, calcium imaging), automated multi-well imaging, slide scanning and deconvolution.

In widefield microscopy cameras are used to capture images without application of advanced imaging techniques to improve axial resolution. This has advantages of being easier, quicker, less damaging and cheaper. Some of our newer systems allow large scale automated image capture for multi-well plate scanning or automated imaging of sections (slide scanning).

Features

Most of our widefield systems have motorised stages for multi-site imaging, adaptive focus control,  some DIC and phase contrast lenses, sensitive monochrome cameras, incubators for temperature control and CO₂ enrichment if required for live cell imaging.

Widefield 5 and the Evident VS200 slides scanner have additional colour cameras – e.g. for histological staining.

Widefield 6 has a Photometrics Prime 95B sCMOS camera for sensitive high speed live cell imaging as well as improved resolution techniques such as super resolution radial fluctuation microscopy (SRRF).