The Wolfson Bioimaging Facility has 6 widefield microscopes offering routine fluorescence and transmitted light imaging alongside advanced applications including ratiometric analysis (e.g. FRET, calcium imaging), multi-well imaging and deconvolution.

In widefield microscopy cameras are used to capture images equivalent to the view down the microscope eyepieces. When the additional features of more advanced imaging techniques such as CLSM (e.g. improved axial resolution and region of interest scanning) are not essential, use of widefield microscopes should be considered as they are easier, quicker and cheaper. Some of our newer systems allow large scale automated image capture.


All our widefields except Widefield 2 have:

  • inverted microscopes with some DIC and phase contrast lenses
  • sensitive monochrome cameras
  • incubators for temperature control
  • CO2 enrichment if required for live cell imaging.

Widefield systems 5, 6, and 7 all have motorised stages for multi-site imaging.

Widefield system 5 has a colour CCD camera (it also has a monochrome CCD camera on a separate port).

Widefield systems 5, 6 and 7 have adaptive focus control to correct for focus drift during time-lapse imaging.

Widefield 6 has a Photometrics Prime 95B sCMOS camera for sensitive high speed live cell imaging as well as improved resolution techniques such as super resolution radial fluctuation microscopy (SRRF).

Full list of equipment
SystemRoomFurther information

Widefield 1
Dual camera system with fast excitation and emission wavelength and focus control


Widefield 1 technical specifications (PDF, 237kB)

User guide (PDF, 1,079kB)

Widefield 2
WFM with Leica DFC365FX camera

(restricted availability)

 B10A Widefield 2 technical specifications (PDF, 176kB)
Widefield 5
WFM with motorised stage, adaptive focus control, Leica DFC365FX and  DFC420C colour camera
B10A Widefield 5 technical specifications (PDF, 202kB)
Widefield 6
WFM with motorised stage, adaptive focus control, Photometrics Prime 95B sCMOS camera
B10A Widefield 6 technical specifications (PDF, 152kB)
Widefield 7
WFM with motorised stage, adaptive focus control, Andor Ixon EMCCD and Neo sCMOS cameras
C40A  Widefield 7 technical specifications (PDF, 214kB)

IncuCyte S3



Celldiscoverer 7 – automated widefield system

B10C Celldiscoverer 7 technical specifications (PDF, 172kB)

Evident (Olympus) VS200 slide scanner 

C40 Slide scanner technical specifications (PDF, 164kB)Slide scanner technical specifications (PDF, 164kB)Slide scanner technical specifications (PDF, 164kB)

More information and access

For further information or to arrange access to this equipment, contact one of the team.

We welcome comments or suggestions. Please contact one of the team or one of the advisory group.

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