The Wolfson Bioimaging Facility has 6 widefield microscopes offering routine fluorescence and transmitted light imaging alongside advanced applications including ratiometric analysis (e.g. FRET, calcium imaging), multi-well imaging and deconvolution.
In widefield microscopy cameras are used to capture images equivalent to the view down the microscope eyepieces. When the additional features of more advanced imaging techniques such as CLSM (e.g. improved axial resolution and region of interest scanning) are not essential, use of widefield microscopes should be considered as they are easier, quicker and cheaper. Some of our newer systems allow large scale automated image capture.
All our widefields except Widefield 2 have:
- inverted microscopes with some DIC and phase contrast lenses
- sensitive monochrome cameras
- incubators for temperature control
- CO2 enrichment if required for live cell imaging.
Widefield systems 5, 6, and 7 all have motorised stages for multi-site imaging.
Widefield system 5 has a colour CCD camera (it also has a monochrome CCD camera on a separate port).
Widefield systems 5, 6 and 7 have adaptive focus control to correct for focus drift during time-lapse imaging.
Widefield 6 has a Photometrics Prime 95B sCMOS camera for sensitive high speed live cell imaging as well as improved resolution techniques such as super resolution radial fluctuation microscopy (SRRF).
|B10A||Widefield 2 technical specifications (PDF, 176kB)|
WFM with motorised stage, adaptive focus control, Leica DFC365FX and DFC420C colour camera
|B10A||Widefield 5 technical specifications (PDF, 202kB)|
WFM with motorised stage, adaptive focus control, Photometrics Prime 95B sCMOS camera
|B10A||Widefield 6 technical specifications (PDF, 152kB)|
WFM with motorised stage, adaptive focus control, Andor Ixon EMCCD and Neo sCMOS cameras
|C40A||Widefield 7 technical specifications (PDF, 214kB)|
Celldiscoverer 7 – automated widefield system
|B10C||Celldiscoverer 7 technical specifications (PDF, 172kB)|
Evident (Olympus) VS200 slide scanner
|C40||Slide scanner technical specifications (PDF, 164kB)Slide scanner technical specifications (PDF, 164kB)Slide scanner technical specifications (PDF, 164kB)|