The Wolfson Bioimaging Facility supports state-of-the-art multiphoton imaging with its multi-laser Leica MP/CLSM system.

Multiphoton (MP) microscopy allows imaging deeper within tissues and organisms than our other fluorescence techniques. MP uses two longer wavelength/lower energy photons – which penetrates tissue more readily – to excite fluorophores that are excited at lower wavelengths in single photon techniques such as CLSM.

Excitation requires two photons to be absorbed by the sample almost instantaneously and as power densities are only high enough for this to happen in the focal plane, multiphoton excitation provides inherent optical sectioning. 

MP can be used to image fluorophores, intrinsic fluorescence and, by second harmonic generation, structural features of tissue components such as collagen and muscle.

Our multiphoton systems is a Leica SP8 tandem scanning system with Spectra Physics Deep See (680-1300nm plus 1040nm) laser for multiphoton excitation and additional lasers for single photon excitation (Argon, 561 and 633nm). An array of external non-descanned detectors (2PMT+2 HyD) for MP imaging, internal detectors for CLSM (1PMT+2HyD) and a transmitted light detector provide flexibility over imaging options.

Multiphoton technical specifications (PDF, 242kB)

Recent publications including data acquired with our multiphoton system:

More information and access

For further information or to arrange access to this equipment, contact one of the team.

We welcome comments or suggestions. Please contact one of the team or one of the advisory group.

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