Widefield

The Wolfson Bioimaging Facility has 6 widefield microscopes offering routine fluorescence and transmitted light imaging alongside advanced applications including ratiometric analysis (e.g. FRET, calcium imaging), multi-well imaging and deconvolution.

The Wolfson Bioimaging Facility has a suite of widefield microscopes offering routine fluorescence and transmitted light imaging alongside advanced applications including ratiometric analysis (e.g. FRET, calcium imaging), automated multi-well imaging, slide scanning and deconvolution.

In widefield microscopy cameras are used to capture images without application of advanced imaging techniques to improve axial resolution. This has advantages of being easier, quicker, less damaging and cheaper. Some of our newer systems allow large scale automated image capture for multi-well plate scanning or automated imaging of sections (slide scanning).

Features

Most of our widefield systems have motorised stages for multi-site imaging, adaptive focus control,  some DIC and phase contrast lenses, sensitive monochrome cameras, incubators for temperature control and CO2 enrichment if required for live cell imaging.

Widefield 5 and the Evident VS200 slides scanner have additional colour cameras – e.g. for histological staining.

Widefield 6 has a Photometrics Prime 95B sCMOS camera for sensitive high speed live cell imaging as well as improved resolution techniques such as super resolution radial fluctuation microscopy (SRRF).

Full list of equipment
SystemRoomFurther information

Widefield 1
Dual camera system with fast excitation and emission wavelength and focus control

C27A

Widefield 1 technical specifications (PDF, 237kB)

User guide (PDF, 1,079kB)

Widefield 2
WFM with Leica DFC365FX camera

(restricted availability)

 B10A Widefield 2 technical specifications (PDF, 176kB)
Widefield 5
WFM with motorised stage, adaptive focus control, Leica DFC365FX and  DFC420C colour camera
B10A Widefield 5 technical specifications (PDF, 202kB)
Widefield 6
WFM with motorised stage, adaptive focus control, Photometrics Prime 95B sCMOS camera
B10A Widefield 6 technical specifications (PDF, 152kB)
Widefield 7
WFM with motorised stage, adaptive focus control, Andor Ixon EMCCD and Neo sCMOS cameras
C40A  Widefield 7 technical specifications (PDF, 214kB)

IncuCyte S3

 

 C40 IncuCyte S3 technical specifications (PDF, 128kB)

Celldiscoverer 7 – automated widefield system

B10C Celldiscoverer 7 technical specifications (PDF, 172kB)

Evident (Olympus) VS200 slide scanner 

C40 Slide scanner technical specifications (PDF, 164kB)Slide scanner technical specifications (PDF, 164kB)‌‌‌

Leica MX10 F fluorescence stereomicroscope

B10A Fluorescence Stereomicroscope - technical specifications (PDF, 81kB)Fluorescence Stereomicroscope - technical specifications (PDF, 82kB)

More information and access

For further information or to arrange access to this equipment, contact one of the team.

We welcome comments or suggestions. Please contact one of the team or one of the advisory group.

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