Total internal reflection fluorescence (TIRF)
The Wolfson Bioimaging Facility provides total internal reflection fluorescence microscopy (TIRF) with its multi-laser, dual-camera Olympus/Abbelight SAFe360 system which also offers single molecule super-resolution imaging.
TIRF microscopy provides extremely high axial resolution by exciting the fluorophores with an evanescent field (caused when the illuminating laser reflects off the glass/water or glass/sample interface) rather than a lamp or laser. This evanescent field only penetrates 50-150 nm into the sample providing extremely high signal to noise, as those fluorophores found further than this from the interface are not excited.
Abbelight TIRF + SM technical specifications (PDF, 160kB)
Recent papers:
- Kuzmuk, Pranke, Rollason, Butler, Ding, Beesley, Waters, Coward, Sessions, Tuffin, Foster, Mollet, Antignac, Edelman, Welsh, Saleem. (2024) A small molecule chaperone rescues keratin-8 mediated trafficking of misfolded podocin to correct genetic nephrotic syndrome. Kidney International 105: 744-758
- Lu, Veler, Simonetti, Raj, Chou, Cross, Phillips, Ruan, Huynh, Dowsey, Ye, Murphy, Verkade, Cullen, Wülfing (2023) Five inhibitory receptors display distinct vesicular distributions in murine T cells. Cells 12(21):2558<
- Ding, Kuzmuk, Hunter, Lay, Hayes, Beesley, Rollason, Hurcombe, Barrington, Masson, Cathery, May, Tuffin, Roberts, Mollet, Chu, Mcintosh, Coward, Antignac, Nathwani, Welsh, Saleem (2023) Adeno-associated virus gene therapy prevents progression of kidney disease in genetic models of nephrotic syndrome Science Translational Medicine DOI: 10.1126/scitranslmed.abc8226
More information and access
For further information or to arrange access to this equipment, contact one of the team.
We welcome comments or suggestions. Please contact one of the team or one of the advisory group.
