In situ structural immunology - sub-nm resolution structure determination from minimal systems towards whole cells

13 October 2022, 1.00 PM - 13 October 2022, 2.00 PM

Dr Thom Sharp (Leiden University)

C42 (Biomedical Sciences Building), University of Bristol

Our immune system relies on the transient formation of protein complexes and receptor clustering to regulate activating and inhibitory signals. These interactions are generally lost when performing structural biology on purified proteins. To address this, Thom's team have developed minimal systems to recapitulate native protein-protein interactions, including the use of antigenic cell mimetics and DNA origami platforms to perform both biophysical analysis and structural biology of immune system complexes. Thom will present his latest data on structural biology of immune system components and how these structures are inspiring his team to develop novel activating and inhibiting immunotherapeutics. He will present their latest developments towards super-res-cryoCLEM on cellular samples, which they are now using to perform structural biology of intracellular complexes.

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