Publications

This list contains the publications that originated in the Wülfing lab as well as publications where we have contributed key imaging data.

Edmunds, G. L., Wong, C. W., Ambler, R., Milodowski, E. J., Alamir, H., Cross, S. J., Galea, G., Wülfing, C. and Morgan, D. J. (2022) Adenosine 2A receptor and TIM3 suppress cytolytic killing of tumor cells via cytoskeletal polarization. Comms Biology, 5, Article number 9
Li, Y., Tunbridge, H. M., Britton, G. J., Hill, E.V., Sinai, P., Cirillo, S., Thompson, C., Fallah-Arani, F., Dovedi, S. J.,Wraith, D. C. and Wülfing, C. (2021). A LAT-based signaling complex in the immunological synapse as determined with live cell imaging is less stable in T cells with regulatory capability. Cells, 10, 418
Ambler, R., Edmunds, G. L., Tan, S. L., Cirillo, S., Pernes, J. I., Ruan, X., Huete-Carrasco, J., Wong, C. C. W., Lu, J., Ward, J., Toti, G., Hedges, A. J., Dovedi, S. J., Murphy, R. F., Morgan, D. J. and Wülfing, C. (2020). PD-1 suppresses the maintenance of cell couples between cytotoxic T cells and tumor target cells within the tumor. Sci. Signal., 13, eaau4518
Tan, S.L., Alibhai, D., Cross, S.J., Thompson, H. and Wülfing, C. (2020). Super-resolution imaging of the T cell central supramolecular signaling cluster using stimulated emission depletion microscopy. Bio-Protocol, 10, e3806
Clark, D. J, McMillan, L. E., Tan, S. L., Bellomo, G., Massoué, C., Thompson, H., Mykhaylechko, L., Alibhai, D., Ruan, X., Singleton, K. L., Du, M., Hedges, A. J., Schwartzberg, P. L., Verkade, P., Murphy, R. F., and Wülfing, C. (2019). Transient protein accumulation at the center of the T cell antigen presenting cell interface drives efficient IL-2 secretion. eLife, 8, e45789
Ruan, X., Wülfing, C., Murphy, R. F (2017). Image-based spatiotemporal causality inference for protein signaling networks. Bioinformatics, 33, i217-i224. Bioinformatics Volume 33 Issue 14
Ambler, R., Ruan, X., Murphy, R. F., and Wülfing, C. (CW and RFM are joint last authors)(2017). Systems imaging of the immune synapse. Meth. Mol. Biol., 1584, 409-421. Full text available upon request
Britton, G. J., Ambler, R., Clark, D. J., Hill, E. V., Tunbridge, H. M., McNally, K. E., Burton, B. R., Butterweck, P., Sabatos-Peyton, C., Hampton-O’Neil, L. A., Verkade, P., Wülfing, C., and Wraith, D. C. (CW and DCW are joint last authors) (2017) PKC theta links proximal T cell and Notch signaling through localized regulation of the actin cytoskeleton. eLife, 6, 20003.
Roybal, K. T., Buck, T. E., Ruan, X., Cho, B. H. Clark, D. J., Ambler, R., Tunbridge, H., Zhang, J., Verkade, P., *Wülfing, C., and *Murphy, R. F (*joint last authors) (2016) Computational spatiotemporal analysis identifies WAVE2 and Cofilin as joint central regulators of costimulation-mediated T cell actin dynamics. Sci. Signal., 9, rs3.
Ruffo, E., Malacarne, V., Larens, S.E., Das, R., Patrussi, L., Wülfing, C., Biskup, C., Kapnick, S.M., Verbist, K., Tedrick, P., Schwartzberg, P.L., Baldari, T. C., Rubio, I., Nichols, K. E., Snow, A. L., Baldanzi, G., Graziani, A. (2016) Inhibition of diacylglycerol kinase α restores restimulation-induced cell death and reduces immunopathology in XLP-1. Sci. Transl. Med., 8, 321ra7
Wülfing, C. and Roybal, K.T. (2015) Cellular structures controlling T cell signaling in time and space. In: Ralph A Bradshaw and Philip D Stahl (Editors-in-Chief), Encyclopedia of Cell Biology, Vol 3, Waltham, MA: Academic Press, pp. 677-686.
Roybal, K.T., Mace, E.M., Clark, D.J., Leard, A.D., Herman, A., Verkade, P., Orange, J.S. and Wülfing, C. (2015) Modest interference with actin dynamics in primary T cell activation by antigen presenting cells preferentially affects lamellalsignaling. PLoS ONE 10, e0133231.
Roybal, K.T., Mace, E.M., Mantell, J.M., Verkade, P., Orange, J.S., and Wülfing, C. (2015) Early signaling in primary T cells activated by antigen presenting cells is associated with a deep and transient lamellal actin network. PLoS ONE 10, e0133299.
Sinai, P., Dozmorov, I. M., Song, R., Schwartzberg, P. L., Wakeland, E. K., and Wülfing, C. (2014) T/B cell interactions are more transient in response to weak stimuli in SLE-prone mice. Eur. J. Immunol. 44, 3522-31.
Wülfing, C.,Tunbridge, H. M., and Wraith, D. C. (2014). New inhibitory signaling by CTLA-4. Nat. Immunol. 15, 408-409.
Liang, Y., Cucchetti, M. , Roncagalli, R., Yokosuka, T., Malzac, A., Bertosio, E., Imbert, J., Nijman, I. J., MSuchanek, M., Saito, T., Wülfing, C., Malissen, B., and Malissen, M. (2013) The lymphoid lineage-specific actin-uncapping protein Rltpr is essential for costimulation via CD28 and the development of regulatory T cells. Nat. Immunol. 14, 858-866.
Roybal, K.T., Sinai, P., Murphy, R.F., Verkade, P., and Wülfing, C. (2013) The actin-driven spatiotemporal organization of T cell signaling at the systems scale. Immunol. Rev. 256, 133-147.
Paster, W., Brockmeyer, C, Fu, G., Simister, P, de Wet, B., Martinez, A., Feller, S. M., Wülfing, C., Gascoigne, N. R., and Acuto, O. (2013) GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development. J. Immunol. 190, 3749-3756.
Roybal, K. T. and Wülfing, C. (2013) New TACTICS for finding Numb.Immunol. Cell Biol. 91, 1.
Ksionda, O., Saveliev, A., Köchl, R., Rapley, J., Faroudi, M., Smith-Garvin, J. E., Wülfing, C., Rittinger, K., Carter, T., Tybulewicz, V. L. J. (2012) Mechanism and function of Vav1 localization in TCR signalling. J. Cell Sci. 15, 5302-5314.
Sun, Y. Dandekar, R. D., Mao, Y. S., Yin, H. L. and Wülfing, C. (2011) PIP2 controls T cell activation by regulating T cell rigidity and organization. PLoS ONE. 6(11): e27227.
Singleton, K. L., Gosh, M., Dandekar, R. D., Au-Yeung, B. B., Ksionda, O., Tybulewicz, V. J. L., Altman, A., Fowell, D. J., Wülfing, C. (2011) Itk controls the spatiotemporal organization of T cell activation. Sci. Signal., 4(193), ra66.
Deford-Watts, L. M., Dougall, D. S., Johnson, B. A., Eitson, J. L., Sun, Y., Roybal, K. T., Albanesi, J. P., Wülfing, C., van Oers, N. S. C. (2011) The CD3ζ subunit contains a Phosphoinositide-binding motif that is required for the stable accumulation of TCR-CD3 complex at the immunological synapse. J. Immuno. 186, 6839-6847.
Sinai, P., Nguyen, C., Schatzle, J. D., and Wülfing, C. (2010) Transience in polarization of cytolytic effectors is required for efficient killing and controlled by Cdc42. Proc. Natl. Acad. Sci. USA, 107, 11912-11917.
Roybal, K. T. and Wülfing, C. (2010) Inhibiting the inhibitor of the inhibitor: Blocking PKC-Θ to enhance regulatory T cell function. Sci. Signal., 3(132), pe24.
Sinai, P., Roybal, K. T., and Wülfing, C. (2010) Tentative and transient natural killer cell polarization balances the requirements for discriminatory recognition and cytolytic efficacy. Commun. Integr. Biol., 3(6), 545-548.
Singleton, K. L., Roybal, K. T., Sun, Y., Fu, G., Gascoigne, N. R. J., van Oers, N. S. C., and Wülfing, C. (2009) Spatiotemporal patterning during T cell activation is highly diverse. Sci. Signal., 2(65), ra15.
DeFord-Watts, L. M., Tassin, T. C., Becker, A. M., Medeiros, J. J., Albanesi, J. P., Love, P. E., Wülfing, C., van Oers, N. S. C. (2009) The cytoplasmic tail of the T cell receptor CD3 ε subunit contains a phospholipid-binding motif that regulates T cell functions.J. Immunol., 183, 1055-1064.
Tskvitaria-Fuller, I., Mistry, N., Sun, S., and Wülfing, C. (2007) Protein transduction as a means of effective manipulation of Cdc42 activity in primary T cells. J. immunol. Meth., 319, 64-78, available online 8 Dec 2006, doi:10.1016/j.jim.2006.10.017.
Becker, A. M., DeFord-Watts, L. M., Wülfing, C., and van Oers, N. S. C. (2007) The constitutive tyrosine phosphorylation of CD3ζ results from TCR/MHC interactions that are independent of thymic selection. J. Immunol., 178, 4120-4128.
Tskvitaria-Fuller, I., Seth, A., Mistry, N., Gu, H., Rosen, M. K., and Wülfing, C. (2006) Specific patterns of Cdc42 activity regulate distinct elements of T cell polarization. J. Immunol., 177, 1708-1720.
Singleton, K.L., Parvaze, N., Dama, K. R., Chen, K. S., Jennings, P., Purtic, B., Sjaastad, M. D., Gilpin, C., Davis, M. M., and Wülfing, C. (2006) A large T cell invagination with CD2 enrichment resets receptor engagement in the immunological synapse. J. Immunol., 177, 4402-4413.
Lee, K.-M., Forman, J. P., McNerney, M. E., Stepp, S., Sumalatha, K., Guzior, D., Wei, H., Latchman, Y. E., Sayegh, M. H., Yagita, H., Park, C.-K., Oh, S. B., Wülfing, C., Schatzle, J., Mathew, P. A., Sharpe, A. H., and Kumar, V. (2006) Requirement of homotypic NK cell interaction through 2B4(CD244)/CD48 in the generation of NK effector functions. Blood 107, 3181-3188.
Purtic, B., Pitcher, L. A., van Oers, N. S. C., and Wülfing, C. (2005) T cell receptor (TCR) clustering in the immunological synapse integrates TCR and costimulatory signaling in selected T cells. Proc. Natl. Acad. Sci. USA 102, 2904-2909.
Pitcher, L. A., Mathis, M. A., Young, J. A., DeFord, L. M., Purtic, B., Wülfing, C., van Oers, N. S. C. (2005) The CD3 gamma epsilon/delta epsilon signaling module provides normal T cell functions in the absence of the TCR zeta ITAMs.Eur. J. Immunol. 35, 3643-3654.
Mooney, J. M., Klem, J., Wülfing, C., Mijares, L. A., Schwartzberg, P. L., Bennett, M., and Schatzle, J. D. (2004) The murine NK receptor 2B4 (CD244) exhibits inhibitory function independent of signaling lymphocytic activation molecule-associated protein expression. J. Immunol. 173, 3953-3961.
Wülfing, C., Klem, J., Purtic, B., and Schatzle, J. D. (2003) Stepwise cytoskeletal polarization as a series of checkpoints in innate but not adaptive cytolytic killing. Proc. Natl. Acad. Sci. USA 100, 7767-7772.
Tskvitaria-Fuller, I., Rozelle, A. L., Yin, H. L., and Wülfing, C. (2003) Regulation of sustained actin dynamics by the T cell receptor and costimulation as a mechanism of receptor localization. J. Immunol. 171, 2287-2295.
Burroughs, N. and Wülfing, C. (2002) Differential segregation in a cell-cell contact interface – the dynamics of the immunological synapse. Biophys. J., 83, 1784-96.
Wülfing, C., Tskvitaria-Fuller, I., Burroughs, N., Sjaastad, M. D., Klem, J., and Schatzle, J. D. (2002) Interface accumulation of receptor/ligand couples in lymphocyte activation: Methods, mechanism, and significance. Immunol. Rev. 189, 64-83.