Projected funded by John Templeton Foundation (PIs: Jean Golding & Marcus Pembrey).

Staff employed on this grant: Yaz Iles-Caven, Karen Birmingham, Steve Gregory, Genette Ellis.

This project has two aims: (a) to determine further the ways in which the intergenerational responses of the parents (F1) to cigarette smoke in utero or early childhood may affect various

phenotypic and DNA methylation outcomes in their offspring. (b) To determine the efficacy of using self-completion questionnaires to identify key histories concerning the smoking and stress

backgrounds of the study child’s ancestors and thence explore transgenerational effects in phenotypes and in methylation markers.

Data collection includes: (i) questionnaires to both study parents concerning their own parents/grandparents; (ii) a validation exercise (interviews on a random sample of 100 parents);

(iii) DNA methylation markers and (iv) secondary data analyses