Understanding mechanisms involved in immunosuppression induced by porcine reproductive and respiratory syndrome viruses

About the Project
The porcine reproductive and respiratory syndrome virus (PRRSV) poses a significant threat to the global pig industry, causing reproductive failure, respiratory issues, and immunosuppression. Research suggests that the PRRSV NSP2 protein activates the COX2/PGE2 pathway and is linked to regulatory T (Treg) cell responses, contributing to immunosuppression. In our prior work, we found that the COX2/PGE2 pathway, activated by NSP2, upregulates Treg expansion in a TGF-β dependent manner.

This project aims to investigate the role of the COX2/PGE2 pathway in modulating immune responses in pigs following viral infections. The hypothesis is that PRRSV NSP2 activates the pathway in infected macrophages, leading to TGF-β maturation and Treg cell activation, negatively regulating the immune response. Using RT-qPCR, western blot, ELISA, flow cytometry, and a luciferase-based bioassay, the project seeks to assess pathway activation, explore TGF-β-induced Treg cell responses in vitro, and determine the impact of PRRSV mutations on immune responses in vivo.

The student will gain experiences working across three laboratories (Pirbright, Bristol and BIOTEC (Thailand); and will learn and apply a range of state-of-the-art immunological and molecular virology techniques.

Funded PhD Project (UK Students Only)

Further details and how to apply

Closes Friday, March 22, 2024