Equipment

Liquid Chromatography Mass Spectrometry

Name Description
 Ultra-high resolution and accurate mass instrument used for:
Orbitrap Fusion Lumos Mass Spectrometer (Thermo Scientific)

Operated with up-stream Ultimate 3000 nano-LC system.

Thermo Scientific's most advanced mass spectrometry system.
  • Protein identification from highly complex samples
  • SILAC and phospho-SILAC
  • TMT and phospho-TMT (using SPS-MS3 analysis)
Orbitrap Fusion Tribrid Mass Spectrometer with ETD (Thermo Scientific) Operated with up-stream Ultimate 3000 nano-LC system.
  • Protein identification from highly complex samples
  • SILAC and phospho-SILAC
  • TMT and phospho-TMT (using SPS-MS3 analysis)
LTQ-Orbitrap Velos Mass Spectrometer with ETD (Thermo Scientific) Operated with up-stream Ultimate 3000 nano-LC system.
  • Protein interaction studies (AP-MS, IPs, etc.)
  • Protein identification from less complex samples

MALDI-Mass spectrometry

Name Description
Bruker Daltonics UltrafleXtreme 2 (housed in the School of Chemistry Mass Spectrometry Facility)

High resolution and accurate mass instrument used for:

Protein identification from single gel bands

Automated Proteolytic Digestion

Name Description
DigestPro MSi Robot (Intavis Bioanalytical Instruments) Automates proteolytic digestion to minimise sample handling. 96-well plate format allows the digestion of multiple samples in a single run. 

Liquid Chromatography

Name Description
Ultimate 3000 Bio-RS HPLC system (Thermo Scientific) Used for the fractionation of peptide mixtures using basic RP chromatography, SAX chromatography or SCX chromatography.