Especially suited to viewing very large image stacks (10s to 100s of GB). It has capability for volumetric and surface renders of images/movies. It can be used to segment objects and measure shape descriptors (including volume, surface area and ellipticity). 

Features

• Stitching and alignment of multiple image stacks.
• Built-in modular analysis functionality spanning a wide range of image processing algorithms. These include morphological filtering, denoising, thresholding, and pixel classification.
• Object-based analysis, allowing objects to be identified then quantified in terms of image intensity, size, and shape (amongst others).
• Compatible with Leica .lif files and Zeiss .czi files.
• Python and MATLAB scripting interface.

Advantages

• Highly suited to visualising and processing very large datasets due to high-performance rendering capability.
• Compatible with Zeiss .czi image format produced by lightsheet microscope and Leica .lif format produced by Wolfson Bioimaging confocal and widefield microscopes.
• Compatible with Ilastik image classification models.
• Functionality can be extended through Python and MATLAB scripting interface.

Disadvantages

• Single license available in facility and no free version for home/personal use.

Availability

• Commercial software requiring a license.
• Additional functionality (colocalisation, object tracking and batch processing) requires further module licences.
• Available on 'Stelzer' image processing computer in Phase II lobby area. Access to this computer can be booked on PPMS. Priority is given to lightsheet microscope users.

Additional information

• Arivis Vision4D website 
• Video tutorials