Services and equipment

Access our range of electron cryo-microscopy equipment and benefit from our various services tailored to your needs.

• FEI Talos Arctica
  Our FEI Talos Arctica is equipped with a 200 kV X-FEG, Ceta 16 M CCD detector, Gatan K2 DED, and Gatan GIF Quantum LS energy filter. The microscope is particularly well suited for high-throughput screening and high-resolution data collection. EPU and TOMO software automate single particle and tomography data acquisition, while Relion 4.0 on-the-fly processing gives live feedback on data quality. Aberration free image shift (AFIS) accelerates image acquisition at a rate that is up to four times faster.
• FEI Tecnai 20
  The Wolfson Bioimaging Facility houses an FEI Tecnai T20 equipped with a 200 kV LaB6 thermionic electron source and Eagle 2k CCD detector. It enables ambient temperature and cryo-electron microscopy of single particle or tomography samples to obtain preliminary data prior to more detailed analysis on higher specification TEMs.
• FEI Tecnai 12
  The FEI Tecnai T12 located in the Wolfson Bioimaging Facility and is equipped with a 120 kV tungsten thermionic cathode and Ceta 16 M CCD detector. It is operated at ambient temperatures and allows users to screen and acquire datasets of negative stained samples.

• FEI Vitrobot
  The Vitrobot is a semi-automated cryo-grid plunge-freezing device that offers precise control over critical parameters. Two blotting pads to either side of the specimen remove excess fluid by contacting the bottom edge of the cryo-grid at an angle with filter paper, and typically creates a gradient in ice thickness across the grid.
• Leica EM GP
  The Leica EM GP is located in the Wolfson Bioimaging Facility. It is a semi-automated cryo-grid plunge freezing device that blots excess fluid by contacting the front or back face of the cryo-grid with a filter paper. It offers control over a wide range of blotting parameters.

• ELMO Glow Discharge System
  The ELMOT Glow Discharge System provides users with fine controls to pretreat TEM support films and change surface properties such as hydrophilicity and charge.