Proteomics Facility

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Selected Publications

Here are some examples of recent publications which have included work performed within the facility.

Protein Identification and Protein Interaction studies

  1. Wan Nur Ismah WAK, Takebayashi Y, Findlay J, Heesom KJ, Avison MB. (2018) J Antimicrob Chemother. 1;73(11):2990-2996. Impact of OqxR loss of function on the envelope proteome of Klebsiella pneumoniae and susceptibility to antimicrobials.
  2. Jiménez-Castellanos JC, Wan Nur Ismah WAK, Takebayashi Y, Findlay J, Schneiders T, Heesom KJ, Avison MB. (2018) J Antimicrob Chemother. 1;73(1):88-94. Envelope proteome changes driven by RamA overproduction in Klebsiella pneumoniae that enhance acquired β-lactam resistance.
  3. Wan Nur Ismah WAK, Takebayashi Y, Findlay J, Heesom KJ, Jiménez-Castellanos JC, Zhang J, Graham L, Bowker K, Williams OM, MacGowan AP, Avison MB. (2018) Antimicrob Agents Chemother. 23;62(3). Prediction of Fluoroquinolone Susceptibility Directly from Whole-Genome Sequence Data by Using Liquid Chromatography-Tandem Mass Spectrometry to Identify Mutant Genotypes.
  4. Durrant TN, Hutchinson JL, Heesom KJ, Anderson KE, Stephens LR, Hawkins PT, Marshall AJ, Moore SF, Hers I. (2017) Blood Adv. 13;1(14):918-932. In-depth PtdIns(3,4,5)P3 signalosome analysis identifies DAPP1 as a negative regulator of GPVI-driven platelet function.
  5. Hutchinson D, Clarke A, Heesom K, Murphy D, Eggleton P. (2017) ERJ Open Res. 3(3). Carbamylation/citrullination of IgG Fc in bronchiectasis, established RA with bronchiectasis and RA smokers: a potential risk factor for disease.
  6. Calvopiña K, Hinchliffe P, Brem J, Heesom KJ, Johnson S, Cain R, Lohans CT, Fishwick CWG, Schofield CJ, Spencer J, Avison MB. (2017) Mol Microbiol. 106(3):492-504. Structural/mechanistic insights into the efficacy of nonclassical β-lactamase inhibitors against extensively drug resistant Stenotrophomonas maltophilia clinical isolates.
  7. Clarke A, Perry E, Kelly C, De Soyza A, Heesom K, Gold LI, Ollier W, Hutchinson D, Eggleton P. (2017) Int J Biochem Cell Biol.;89:199-206. Heightened autoantibody immune response to citrullinated calreticulin in bronchiectasis: Implications for rheumatoid arthritis.
  8. Maringer K, Yousuf A, Heesom KJ, Fan J, Lee D, Fernandez-Sesma A, Bessant C, Matthews DA, Davidson AD. (2017) BMC Genomics 18, 101-119. Proteomics informed by transcriptomics for characterising active transposable elements and genome annotation in Aedes aegypti.
  9. Battram AM, Durrant TN, Agbani EO, Heesom KJ, Paul DS, Piatt R, Poole AW, Cullen PJ, Bergmeier W, Moore SF, Hers I. (2016) J. Biol. Chem. 292, 1691-1704. The Phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3)-binder Rasa3 Regulates Phosphoinositide 3-kinase (PI 3-kinase)-dependent Integrin αIIbβ3 Outside-in Signaling.
  10. Jiménez-Castellanos JC, Wan Ahmad Kamil WN, Cheung CH, Tobin MS, Brown J, Isaac SG, Heesom KJ, Schneiders T, Avison MB. (2016) J Antimicrob. Chemother. 71, 1820-1825. Comparative effects of overproducing the AraC-type transcriptional regulators MarA, SoxS, RarA and RamA on antimicrobial drug susceptibility in Klebsiella pneumoniae. 

SILAC

  1. Andrew RJ, Fisher K, Heesom KJ, Kellett KAB, Hooper NM. (2019) J Neurochem. doi: 10.1111/jnc.14666. Quantitative interaction proteomics reveals differences in the interactomes of amyloid precursor protein isoforms.
  2. McGough IJ, de Groot REA, Jellett AP, Betist MC, Varandas KC, Danson CM, Heesom KJ, Korswagen HC, Cullen PJ. (2018) Nat Commun. 9(1):3737. SNX3-retromer requires an evolutionary conserved MON2:DOPEY2:ATP9A complex to mediate Wntless sorting and Wnt secretion.
  3. Greenhough A, Bagley C, Heesom KJ, Gurevich DB, Gay D, Bond M, Collard TJ, Paraskeva C, Martin P, Sansom OJ, Malik K, Williams AC. (2018) EMBO Mol Med. 10(11). pii: e8699. Cancer cell adaptation to hypoxia involves a HIF-GPRC5A-YAP axis.
  4. Thomas EC, Hook SC, Gray A, Chadt A, Carling D, Al-Hasani H, Heesom KJ, Hardie DG, Tavaré JM (2018) Biochem J. 475(18):2969-2983. Isoform-specific AMPK association with TBC1D1 is reduced by a mutation associated with severe obesity.
  5. Danson CM, Pearson N, Heesom KJ, Cullen PJ. (2018) J Cell Sci.131(17). pii: jcs211672. Sorting nexin-21 is a scaffold for the endosomal recruitment of huntingtin.
  6. Simonetti B, Danson CM, Heesom KJ, Cullen PJ. (2017) J Cell Biol. 6;216(11):3695-3712. Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.
  7. McNally KE, Faulkner R, Steinberg F, Gallon M, Ghai R, Pim D, Langton P, Pearson N, Danson CM, Nägele H, Morris LL, Singla A, Overlee BL, Heesom KJ, Sessions R, Banks L, Collins BM, Berger I, Billadeau DD, Burstein E, Cullen PJ. (2017) Nat Cell Biol. (10):1214-1225. Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.
  8. Emmott E, Sorgeloos F, Caddy SL, Vashist S, Sosnovtsev S, Lloyd R, Heesom K, Locker N, Goodfellow I. (2017) Mol. Cell. Proteomics 16 (4 suppl 1), S215-S229. Norovirus-mediated modification of the translational landscape via virus and host-induced cleavage of translation initiation factors.
  9. Kvainickas A, Orgaz AJ, Nägele H, Diedrich B, Heesom KJ, Dengjel J, Cullen PJ, Steinberg F. (2017) J. Cell. Sci. 130, 382-395. Retromer- and WASH-dependent sorting of nutrient transporters requires a multivalent interaction network with ANKRD50.
  10. McMillan KJ, Gallon M, Jellett AP, Clairfeuille T, Tilley FC, McGough I, Danson CM, Heesom KJ, Wilkinson KA, Collins BM, Cullen PJ. (2016) J. Cell. Biol. 214, 389-399. Atypical parkinsonism-associated retromer mutant alters endosomal sorting in specific cargo proteins.

TMT

  1. Morgan RG, Ridsdale J, Payne M, Heesom KJ, Wilson MC, Davidson A, Greenhough A, Davies S, Williams AC, Blair A, Waterman ML, Tonks A, Darley RL. (2019) Haematologica. pii: haematol.2018.202846. LEF-1 drives aberrant β-catenin nuclear localization in myeloid leukemia cells.
  2. Bond AR, Iacobazzi D, Abdul-Ghani S, Ghorbel MT, Heesom KJ, George SJ, Caputo M, Suleiman MS, Tulloh RM. (2019) J Proteomics. 191:107-113. The cardiac proteome in patients with congenital ventricular septal defect: A comparative study between right atria and right ventricles.
  3. Ion R, Hudson C, Johnson J, Yuan W, Heesom K, López Bernal A. (2018) Reprod Toxicol. 82:18-24. Smoking alters hydroxyprostaglandin dehydrogenase expression in fetal membranes.
  4. Redondo J, Sarkar P, Kemp K, Heesom KJ, Wilkins A, Scolding NJ, Rice CM. (2018) Stem Cells Transl Med. 7(10):748-758. Dysregulation of Mesenchymal Stromal Cell Antioxidant Responses in Progressive Multiple Sclerosis.
  5. Black VL, Heesom K, Whittington F, Davis S, Tasker S, Adamantos S, Davidson AD. (2018) Vet Clin Pathol. 47(3):377-385. Assessment of the red blood cell proteome in a dog with unexplained hemolytic anemia.
  6. Yokoyama M, Stevens E, Laabei M, Bacon L, Heesom K, Bayliss S, Ooi N, O'Neill AJ, Murray E, Williams P, Lubben A, Reeksting S, Meric G, Pascoe B, Sheppard SK, Recker M, Hurst LD, Massey RC. (2018) Genome Biol. 19(1):94. Epistasis analysis uncovers hidden antibiotic resistance-associated fitness costs hampering the evolution of MRSA.
  7. Hawksworth J, Satchwell TJ, Meinders M, Daniels DE, Regan F, Thornton NM, Wilson MC, Dobbe JG, Streekstra GJ, Trakarnsanga K, Heesom KJ, Anstee DJ, Frayne J, Toye AM (2018) EMBO Mol Med. 10(6). pii: e8454. Enhancement of red blood cell transfusion compatibility using CRISPR-mediated erythroblast gene editing.
  8. Bond AR, Iacobazzi D, Abdul-Ghani S, Ghorbel M, Heesom K, Wilson M, Gillett C, George SJ, Caputo M, Suleiman S, Tulloh RMR. (2018) Open Heart. 5(1):e000716. Changes in contractile protein expression are linked to ventricular stiffness in infants with pulmonary hypertension or right ventricular hypertrophy due to congenital heart disease.
  9. Trakarnsanga K, Griffiths RE, Wilson MC, Blair A, Satchwell TJ, Meinders M, Cogan N, Kupzig S, Kurita R, Nakamura Y, Toye AM, Anstee DJ, Frayne J. (2017) Nature Communications 8, 14750.  An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.
  10. Wilson MC, Trakarnsanga K, Heesom KJ, Cogan N, Green C, Toye AM, Parsons SF, Ansteee DJ, Frayne J. (2016) Mol. Cell. Proteomics 15, 1938-1946.  Comparison of the proteome of adult and cord erythroid cells, and changes in the proteome following reticulocyte maturation.

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