Liquid Chromatography Mass Spectrometry

Orbitrap Fusion Lumos Mass Spectrometer (Thermo Scientific)

Operated with up-stream Ultimate 3000 nano-LC system.

Thermo Scientific's most advanced mass spectrometry system.

Ultra-high resolution and accurate mass instrument used for:

• Protein identification from highly complex samples
• SILAC and phospho-SILAC
• TMT and phospho-TMT (using SPS-MS3 analysis)

Orbitrap Fusion Tribrid Mass Spectrometer with ETD (Thermo Scientific)

Operated with up-stream Ultimate 3000 nano-LC system.

Ultra-high resolution and accurate mass instrument used for:

• Protein identification from highly complex samples
• SILAC and phospho-SILAC
• TMT and phospho-TMT (using SPS-MS3 analysis)

LTQ-Orbitrap Velos Mass Spectrometer with ETD (Thermo Scientific)

Operated with up-stream Ultimate 3000 nano-LC system.

Ultra-high resolution and accurate mass instrument used for:

• Protein interaction studies (AP-MS, IPs, etc.)
• Protein identification from less complex samples

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MALDI-Mass spectrometry

Bruker Daltonics UltrafleXtreme 2 (housed in the School of Chemistry Mass Spectrometry Facility)

High resolution and accurate mass instrument used for:

• Protein identification from single gel bands

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Automated Proteolytic Digestion

DigestPro MSi Robot (Intavis Bioanalytical Instruments)

Automates proteolytic digestion to minimise sample handling. 96-well plate format allows the digestion of multiple samples in a single run.

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Liquid Chromatography

Ultimate 3000 Bio-RS HPLC system (Thermo Scientific)

Used for the fractionation of peptide mixtures using basic RP chromatography, SAX chromatography or SCX chromatography.