Next generation sequencing and library preparation
The Bristol Genomics Facility has the capacity to run both high output and low output next generation sequencing experiments.
The Illumina NextSeq500 system is perfect for the sequencing of whole genomes and transcriptomes, and the Illumina MiSeq has a range of outputs and read lengths generally suited to the sequencing of small genomes, amplicons and targeted applications. Both instruments offer a rapid run time ensuring quick delivery of results. We offer a range of library preparation services and are happy to consider custom library preparation options to assist your research.
Library preparation services
- DNA Library Preparation
- Stranded total RNA and mRNA Library Preparation
- NGS-based amplicon sequencing
- ITS sequencing to identify fungi
- 16S rRNA sequencing to characterise bacterial communities
- Amplicon sequencing with custom primers
Illumina MiSeq sequencing
- Version 3 reagents
- Paired end 2 x 300bp
- Paired end 2 x 75bp
- Version 2 reagents
- Nano, paired-end
- Micro, paired-end
Illumina NextSeq500 sequencing
- High Output Version 2.5
- Paired end 2 x 150bp
- Paired end 2 x 75bp
- Single end 1 x 75bp
- Mid Output Version 2.5
- Paired end 2 x 150bp
- Paired end 2 x 75bp
Illumina MiSeq instrument – for small genomes, amplicons and targeted applications requiring lower output
Illumina NextSeq instrument – for whole genomes and transcriptomes requiring high output
Automated liquid handling capabilities for library preparation